SrasV1, Main, Exploration, bibRecord, 003748

Comparison of immunoglobulin G responses to the spike and nucleocapsid proteins of severe acute respiratory syndrome (SARS) coronavirus in patients with SARS.

Identifieur interne : 003748 ( Main/Exploration ); précédent : 003747; suivant : 003749

Comparison of immunoglobulin G responses to the spike and nucleocapsid proteins of severe acute respiratory syndrome (SARS) coronavirus in patients with SARS.

Auteurs : Jincun Zhao [République populaire de Chine] ; Wei Wang ; Wenling Wang ; Zhendong Zhao ; Yan Zhang ; Ping Lv ; Furong Ren ; Xiao-Ming Gao

Source :

Clinical and vaccine immunology : CVI [ 1556-6811 ] ; 2007.

RBID : pubmed:17475765

Descripteurs français

KwdFr :
- Anticorps antiviraux (sang), Immunoglobuline G (sang), Protéines nucléocapside (immunologie), Protéines recombinantes (immunologie), Spécificité des anticorps, Syndrome respiratoire aigu sévère (immunologie), Test ELISA, Tests de neutralisation, Virus du SRAS (immunologie).
MESH :
- immunologie : Protéines nucléocapside, Protéines recombinantes, Syndrome respiratoire aigu sévère, Virus du SRAS.
- sang : Anticorps antiviraux, Immunoglobuline G.
- Spécificité des anticorps, Test ELISA, Tests de neutralisation.

English descriptors

KwdEn :
- Antibodies, Viral (blood), Antibody Specificity, Enzyme-Linked Immunosorbent Assay, Immunoglobulin G (blood), Neutralization Tests, Nucleocapsid Proteins (immunology), Recombinant Proteins (immunology), SARS Virus (immunology), Severe Acute Respiratory Syndrome (immunology).
MESH :
- chemical , blood : Antibodies, Viral, Immunoglobulin G.
- chemical , immunology : Nucleocapsid Proteins, Recombinant Proteins.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome.
- Antibody Specificity, Enzyme-Linked Immunosorbent Assay, Neutralization Tests.

Abstract

Both the nucleocapsid (N) and the spike (S) proteins of severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) are able to induce strong humoral responses in humans following an infection. To compare the immunoglobulin G (IgG) responses to the S and N proteins of SARS-CoV in SARS patients during the manifestation/convalescent period with those during the postinfection period, serum samples were collected from hospitalized SARS patients within 6 weeks after the onset of illness (set 1; 57 sequential samples from 19 patients) or 2 to 3 months after their recovery (set 2; 33 postinfection samples from 33 subjects). Serum samples from 100 healthy blood donors (set 3), collected in 2002, were also included. The specific IgG response to whole virus, the fragment from positions 450 to 650 of the S protein (S450-650), and the full-length N protein of SARS-CoV were measured by enzyme-linked immunosorbent assays (ELISAs). Western blot assays were carried out to confirm the ELISA results. Fifty-one of the serum samples in set 1 (89%) bound to the N protein, a proportion similar to that which recognized whole virus (79%) and the S-protein fragment (77%). All 33 serum samples from set 2 were strongly positive for N-protein-specific IgG, while 27 (82%) were positive for anti-S450-650 IgG. Two of the serum samples from set 3 were strongly positive for anti-N-protein IgG but not anti-S450-650 IgG. Similar levels of IgG responses to the S and N proteins were observed in SARS patients during the manifestation and convalescent stages. In the postinfection period, however, a number of patients had much lower serum IgG levels against S450-650 than against the N protein.

DOI: 10.1128/CVI.00432-06
PubMed: 17475765

Affiliations:

Le document en format XML

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<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Immunoglobulin G (blood)</term>
<term>Neutralization Tests</term>
<term>Nucleocapsid Proteins (immunology)</term>
<term>Recombinant Proteins (immunology)</term>
<term>SARS Virus (immunology)</term>
<term>Severe Acute Respiratory Syndrome (immunology)</term>
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<term>Immunoglobuline G (sang)</term>
<term>Protéines nucléocapside (immunologie)</term>
<term>Protéines recombinantes (immunologie)</term>
<term>Spécificité des anticorps</term>
<term>Syndrome respiratoire aigu sévère (immunologie)</term>
<term>Test ELISA</term>
<term>Tests de neutralisation</term>
<term>Virus du SRAS (immunologie)</term>
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<term>Recombinant Proteins</term>
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<term>Protéines recombinantes</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Virus du SRAS</term>
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<term>Severe Acute Respiratory Syndrome</term>
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<term>Immunoglobuline G</term>
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<keywords scheme="MESH" xml:lang="en"><term>Antibody Specificity</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Neutralization Tests</term>
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<term>Tests de neutralisation</term>
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<front><div type="abstract" xml:lang="en">Both the nucleocapsid (N) and the spike (S) proteins of severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) are able to induce strong humoral responses in humans following an infection. To compare the immunoglobulin G (IgG) responses to the S and N proteins of SARS-CoV in SARS patients during the manifestation/convalescent period with those during the postinfection period, serum samples were collected from hospitalized SARS patients within 6 weeks after the onset of illness (set 1; 57 sequential samples from 19 patients) or 2 to 3 months after their recovery (set 2; 33 postinfection samples from 33 subjects). Serum samples from 100 healthy blood donors (set 3), collected in 2002, were also included. The specific IgG response to whole virus, the fragment from positions 450 to 650 of the S protein (S450-650), and the full-length N protein of SARS-CoV were measured by enzyme-linked immunosorbent assays (ELISAs). Western blot assays were carried out to confirm the ELISA results. Fifty-one of the serum samples in set 1 (89%) bound to the N protein, a proportion similar to that which recognized whole virus (79%) and the S-protein fragment (77%). All 33 serum samples from set 2 were strongly positive for N-protein-specific IgG, while 27 (82%) were positive for anti-S450-650 IgG. Two of the serum samples from set 3 were strongly positive for anti-N-protein IgG but not anti-S450-650 IgG. Similar levels of IgG responses to the S and N proteins were observed in SARS patients during the manifestation and convalescent stages. In the postinfection period, however, a number of patients had much lower serum IgG levels against S450-650 than against the N protein.</div>
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<country name="République populaire de Chine"><noRegion><name sortKey="Zhao, Jincun" sort="Zhao, Jincun" uniqKey="Zhao J" first="Jincun" last="Zhao">Jincun Zhao</name>
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Comparison of immunoglobulin G responses to the spike and nucleocapsid proteins of severe acute respiratory syndrome (SARS) coronavirus in patients with SARS.

Comparison of immunoglobulin G responses to the spike and nucleocapsid proteins of severe acute respiratory syndrome (SARS) coronavirus in patients with SARS.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

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